Poster
Detecting changes in phosphorylation events during the cell cycle with AlphaLISA SureFire Ultra technology. – Technical Note
Lamin A/C is a nuclear protein involved in multiple cellular processes including chromosome organization, transcriptional regulation, DNA repair, cell signalling and cell cycle regulation1. Lamin A/C phosphorylation plays a role in its incorporation into the nuclear lamina as well as disassembly during mitosis2. Phosphorylation at the so-called “mitotic sites” (Ser22 and Ser392) reaches the maximum level at the onset of mitosis and is triggered by the complex formed by CDK1 and Cyclin B1. The phosphorylation/dephosphorylation events surrounding the CDK1-Cyclin B1-Lamin A/C pathway are associated with alterations in the cell cycle that have an impact not only on the cell morphology and structure but also on the cell fate3. The dysregulation in the levels of these phosphorylated proteins and their total counterparts has been associated with increased cell growth, genomic instability and cell senescence in different types of cancers, becoming a key area for the discovery of efficient therapies to prevent tumor progression1,2