Products

The AlphaLISA™ SureFire® Ultra™ assay enables the rapid and sensitive detection of total and phosphorylated cellular proteins. AlphaLISA® assays utilize two bead types: Acceptor Beads and Donor Beads. The Acceptor Bead is coated with a proprietary CaptSure™ agent to specifically immobilize the assay specific antibody which is labeled with a CaptSure™ tag. The Donor Bead is coated with streptavidin to capture the biotinylated antibody.  

In the presence of a target protein, the two target-specific antibodies bring Donor and Acceptor Beads into close proximity. When the Donor Beads are activated by a laser (680 nm), singlet oxygen is transferred to the Acceptor Bead leading to the production of an Alpha signal. The amount of light emission (615 nm) from the Acceptor Bead is directly proportional to the amount of target protein present in the sample. If an Acceptor Bead is not in close proximity (i.e. 200 nm) of a Donor bead, little to no signal is produced over background. 

This assay eliminates the need for laborious techniques, such as Western blotting or conventional ELISA. It is a homogeneous assay format with many impressive attributes, including: 

• Very simple workflow 
• No washing 
• Short incubation times (2 x 1 h standard format) 
• Large assay dynamic range 
• Enhanced signal-to-noise windows and sensitivity 
• Excellent well-to-well reproducibility (lower CV%) 
• Highly amenable to automation and high throughput screening in 384/1536 well formats.